background: fasciola hepatica and f. gigantica are the causative agents of fasciolosis in domestic animals and humans. based on the morphometric criteria, differential diagnosis between them is problematic. in addition, intermediate forms of fasciola have been found in iran, which makes the differentiation more difficult. the aim of the present study was to provide molecular evidence for the ex...

Rhein (4,5-dibydroxyanthraquinone-Z-carboxylic acid) inhibits the mitochondrial oxidation of reduced nicotamide adenine dinucleotide (NADH) but not of succinate. The inhibition is competitive with respect to substrate and involves a block between NADH and the flavin of NADH dehydrogenase. The Ki is about 2 ~.LM at 30”. Evidence for this localization has come from kinetic analysis of the effect ...

We studied the effects of mexidol (3-oxy-6-methyl-2-ethylpiridine succinate) on antioxidant glutathione system in rat brain mitochondria experimental Parkinson’s disease induced by rotenone administration. Wistar rats were divided into following groups 6 each: I - intact (control); II (3 mg/kg per day) was injected subcutaneously for 2 weeks; III after intoxication, (50 intraperitoneally weeks....

NADH dehydrogenase of Escherichia coli, a component of the electron transport chain, oxidizes NADH to NAD. Previously, it was demonstrated that when E. coli K12 cells were lysed by the lysozyme lysis method, NADH dehydrogenase activity was inhibited; however, when cells were lysed by French press, NADH dehydrogenase activity was observed. In this study, components commonly used in lysozyme lysi...

On the addition of NADH to submitochondrial particles in which NADH oxidase is blocked by rotenone, piericidin A, or Amytal, the g = 1.94 signal of NADH dehydrogenase appears in essentially the same manner as in untreated preparations. However, the appearance of the NADHinduced iron signal of succinate dehydrogenase and of cytochromes b and cl is inhibited by these agents. It is concluded that ...

The specific activities of isocitric dehydrogenase, alpha-ketoglutaric dehydrogenase, succinic dehydrogenase, malic dehydrogenase, and reduced nicotinamide adenine dinucleotide (NADH) oxidase were determined in extracts of Nitrosomonas europaea and compared with the corresponding values for Anacystis nidulans and autotrophically grown Hydrogenomonas eutropha. In common with other obligate autot...

The identification of the efficiency of some mtDNA genes of Mus musculus species complex (house mouse) for biosystematics research was studied in this approach. Recent studies have made use of different mitochondrial genes including NADH dehydrogenase genes, cytochrome b gene, cytochrome oxidase genes, D-loop region and whole mtDNA genome to study the house mouse species. Usage of each of these...

A NADH dehydrogenase was isolated from an inner membrane-enriched fraction of beetroot mitochondria (Beta vulgaris L.) by solubilization with sodium deoxycholate and purified using gel filtration and affinity chromatography. The NADH dehydrogenase preparation contained a minor ATPase contamination. Beetroot mitochondria were chosen as the isolation material for purifying the enzymes responsible...

Conidia of Verticillium albo-atrum Reinke and Berthold, collected from shake cultures grown in Czapek broth, were sonified for 4 or 8 minutes or ground frozen in a mortar to obtain cell-free homogenates. These were assayed for certain enzymes associated with respiratory pathways. Malic dehydrogenase was the most active, glucose-6-P and NADH dehydrogenase were less active, NADH-cytochrome c redu...